Targeted chromosome elimination from ES-somatic hybrid cells

Targeted gene trapping in mouse ES cells

Roland Friedel et al. report a method allowing efficient generation of targeted gene knockouts by homologous recombination in mouse ES cells. Current gene targeting techniques use vectors with a strong promoter to drive expression of a selectable antibiotic resistance marker, but such methods usually yield low homologous recombination frequencies because nonhomologous insertions (false positive...

Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells

Embryonic trisomy leads to abortion or congenital genetic disorders in humans. The most common autosomal chromosome abnormalities are trisomy of chromosomes 13, 18, and 21. Although alteration of gene dosage is thought to contribute to disorders caused by extra copies of chromosomes, genes associated with specific disease phenotypes remain unclear. To generate a normal cell from a trisomic cell...

I-31: Reconstruction of Human Endometrium from Somatic Stem Cells

s:3115:"Background: During reproductive life, the human endometrium undergoes around 500 cycles of growth, breakdown and regeneration. This outstanding regenerative capability is the basis for cyclic endometrial preparation and its dysfunction is involved in pathological disorders. Endometrial regeneration is mediated by the existence of a specialized endometrial stem cell (SC) population recen...

Elimination of chromosome aberrations from human lymphocytes.

Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells

The resetting of a somatic epigenotype to a totipotential state has been demonstrated by successful animal cloning, via transplantation of somatic nuclei into enucleated oocytes. We have established an experimental system, which reproduces the nuclear reprogramming of somatic cells in vitro by fusing adult thymocytes with embryonic stem (ES) cells. Analysis of the lymphoid-cell-specific V-(D)-J...